人单核细胞和T细胞在体外对巨核细胞生成的调节作用

• 作 者: 徐文玉 AlanM.Gewirtz
• 机 构: 上海市免疫学研究所免疫Ⅰ室,上海第二医科大学免疫学教研室 Alan M.Gewirtz Department of Medicine and Thromboals Research Center, T
• 出 处: 上海免疫学杂志
• 发表日期: 1988年01期
• 关键词: 巨核细胞 集落形成单位 血浆凝块 外周血单核细胞 血小板糖蛋白 抗血清 体外培养 巨噬细胞 抑制率 效靶比

已经证明骨髓中的单核一巨噬细胞和T淋巴细胞在体外能调节巨核细胞的生成。本文报告的是外周血单核细胞和T淋巴细胞在体外培养中能影响巨核细胞的集落形成单位(CFU-M)。使用血浆凝块培养技术,抗血小板糖蛋白抗血清作为探针的间接免疫荧光方法以及共培养研究证实粘附单核细胞与自身缺失粘附单核细胞的靶细胞共培养时能抑制CFU-M。与靶细胞单独培养时所形成的CFU-M相比较,其抑制率从26％～46％。而加入T淋巴细胞后,在同样的效靶比例时,对CFU-M没有明显的影响。集落大小检查表明了在外周血中所形成的集落体积大于在骨髓中所形成的集落。

Mononuclear macrophages and T lymphocytes in the bone marrow have been shown to regulate the production of megakaryocytes in vitro. This paper reports the effects of peripheral blood mononuclear cells and T lymphocytes on megakaryocyte colony forming units (CFU-M) in vitro. Indirect immunofluorescence using anti-platelet glycoprotein antiserum as a probe and co-culture studies demonstrated that co-culture of adherent monocytes with target cells lacking adherent monocytes inhibits CFU-M . Compared with the CFU-M formed when the target cells were cultured alone, the inhibition rate was from 26% to 46%. After addition of T lymphocytes, there was no significant effect on CFU-M at the same target ratio. Colony size examination showed that the colony volume formed in the peripheral blood was larger than that formed in the bone marrow.