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目的建立离子色谱同时测定水源水和饮用水中草甘膦和氨甲基膦酸的分析方法。方法水样用0.22μm滤膜过滤后直接进样。色谱条件:Ion Pac AS19色谱柱(250 mm×2 mm)和Ion Pac AG19保护柱(50 mm×2 mm);RFIC系统的EG50淋洗液自动发生器在线产生的KOH作为淋洗液,浓度梯度淋洗,淋洗液流速:0.25 ml/min;抑制器采用外加水模式,电导检测器检测。结果草甘膦和氨甲基膦酸的浓度为0.10 mg/L~2.0 mg/L时,线性关系良好,草甘膦的线性方程为y=0.736 9x+0.003 41,r=0.999 8,检出限和定量限分别为0.004 4 mg/L和0.015 mg/L;氨甲基磷酸的线性方程为y=1.816 4x-0.092 4,r=0.999 6,检出限和定量限分别为0.017 mg/L和0.056 mg/L。在0.20 mg/L、0.60 mg/L、1.00 mg/L 3种加标水平下,草甘膦、氨甲基膦酸的回收率分别为86.3%~98.7%、94.6%~98.1%,相对标准偏差(RSD)为0.42%~5.63%。结论本方法灵敏度和回收率高,选择性好,适用于水源水和饮用水中草甘膦和氨甲基膦酸的测定。
Objective To establish an analytical method for simultaneous determination of glyphosate and aminomethylphosphonic acid in drinking water and drinking water by ion chromatography. Methods Water samples were filtered with 0.22μm filter directly injected. Chromatographic conditions: Ion Pac AS19 column (250 mm × 2 mm) and Ion Pac AG19 guard column (50 mm × 2 mm); KOH generated on-line with the EG50 eluent generator from the RFIC system as a concentration gradient Rinse, eluent flow rate: 0.25 ml / min; suppressor using an external water mode, conductivity detector detection. Results When the concentrations of glyphosate and aminomethylphosphonic acid were between 0.10 mg / L and 2.0 mg / L, the linear relationship was good. The linear equation of glyphosate was y = 0.736 9x + 0.003 41 and r = 0.999 8. The limits of quantification were 0.004 4 mg / L and 0.015 mg / L, respectively. The linear equation of aminomethyl phosphoric acid was y = 1.816 4x-0.092 4, r = 0.999 6. The limits of detection and quantification were 0.017 mg / L And 0.056 mg / L. The recoveries of glyphosate and aminomethylphosphonic acid were 86.3% ~ 98.7% and 94.6% ~ 98.1% at 0.20 mg / L, 0.60 mg / L and 1.00 mg / L respectively. Deviation (RSD) is 0.42% ~ 5.63%. Conclusion The method has high sensitivity and recovery, good selectivity and is suitable for the determination of glyphosate and aminomethylphosphonic acid in drinking water and drinking water.