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通过对松毛虫赤眼蜂TrichogrammadendrolimiMatsumura和玉米螟赤眼蜂T .ostriniaePangetChen(膜翅目 :赤眼蜂科 )核内可转录第二间隔区 (简称 :ITS2 )的克隆、测序 ,并获取和分析了GenBank中已登录的同源序列 ,然后设计了松毛虫赤眼蜂的特异引物以用于该蜂的分子鉴定和检测。经过反复筛选发现 :采用鉴定引物通过PCR扩增不仅可以区分多头样品、单头样品 (雌蜂或雄峰 ) ,而且可鉴定幼期虫态和卵 ,这用传统方法是无法办到的。该鉴定技术比基于形态学鉴定方法优越之处在于 :它不需要专家的介入就能高通量地鉴定单个或残缺、痕量的样品。由该方法衍变成的分子检测技术用来鉴定了从中国大陆不同地域和寄主上采集到的 1 2个样品。结果表明 :该方法可用于赤眼蜂田间分子监测和实验室拟寄生行为研究
Through the cloning, sequencing, and sequencing of the nuclear transcribable second spacer (Trichogramma dendrolimi Matsumura) and the T. ostriniaePangetChen (Hymenoptera: Trichogramma) from the Trichogramma Trichogramma, GenBank has been registered homologous sequences, and then designed a specific primer Trichogramma Trichogramma for the bee molecular identification and detection. After repeated screening, it was found that using primers to amplify by PCR can not only distinguish between multi-head samples and single-head samples (female or male), but also identify young and adult worms and eggs, which can not be achieved by traditional methods. This technique is superior to morphometric methods in that it allows for the high-throughput identification of single or incomplete, trace samples without the need for expert intervention. The molecular detection technique developed by this method was used to identify 12 samples collected from different regions and hosts in mainland China. The results show that this method can be applied to the molecular monitoring of Trichogramma and its parasitism in laboratory