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目的:注射溶血卵磷脂(lysolecithin,LPC)至小鼠颈段脊髓后索诱导轴突脱髓鞘的模型建立及评价体系。方法:手术显微镜下,使用微量注射器将0.4μl 1%LPC注射至成年小鼠第5颈髓(C5)后索。1周后处死动物,取C4～C6颈髓节段,行石蜡切片、甲苯胺蓝染色观察脱髓鞘区域,采用Amira 4.1.1软件三维重建和测量脱髓鞘区域体积,并进行电镜观察轴突脱髓鞘病变。结果:注射LPC造模,小鼠苏醒后无明显运动及感觉异常,甲苯胺蓝染色显示注射区出现体积为(1.41±0.05)×106μm3淡蓝或白色脱髓鞘病变区,电镜结果证实注射区轴突发生脱髓鞘样变。结论:小鼠颈髓后索注射LPC可稳定诱导轴突脱髓鞘。
OBJECTIVE: To establish a model of axonal demyelination induced by injection of lysolecithin (LPC) into the cervical spinal cord of mice and to evaluate the mechanism. Methods: Under a surgical microscope, 0.4 μl of 1% LPC was injected into the fifth cervical spinal cord (C5) of adult mice using a micropipette. One week later, the animals were sacrificed, C4 ~ C6 cervical spinal cord segments were taken, paraffin sections were made and the demyelinated areas were observed with toluidine blue staining. The volume of demyelinating area was reconstructed and measured by Amira 4.1.1 software. Demyelinating myelopathy. Results: After LPC injection, there was no obvious movement and sensory abnormalities in mice after awakening. Toluidine blue staining showed that the area of (1.41 ± 0.05) × 106μm3 pale blue or white demyelinating lesions appeared in the injection zone. Electron microscopy confirmed that the injection zone Axon demyelination degeneration. Conclusion: Injection of LPC into the cervical spinal cord can stably induce axonal demyelination.