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本文应用 ATP 酶细胞化学技术,观察豚鼠皮肤连续涂抹不同浓度原发刺激物新洁而灭(BB)或低浓度致敏原二硝基氯苯(DNCB)后表皮中郎格罕细胞(LC)的动态变化。发现两种刺激物所致的 LC 的密度和形态变化有明显的差异。5%BB 引起可逆性的 LC 密度下降和细胞形态改变。0.05%DNCB 则在涂抹后期引起 LC 的减少和 ATP 酶活性的减弱。本文进一步讨论了以表皮 LC 形态和密度的变化为指标,在评价化妆品安全检测中弱致敏原或弱原发刺激物的意义。
In this paper, ATPase cytochemistry was used to observe the expression of Langerhans cells (LC) in the epidermis of guinea pigs after continuous application of different concentrations of primary stimulants (BB) or low concentrations of dinitrochlorobenzene (DNCB) Dynamic changes. There was a clear difference in the density and morphology of the LCs caused by the two stimuli. 5% BB caused a reversible decrease in LC density and changes in cell morphology. 0.05% DNCB caused a decrease in LC and a decrease in ATPase activity late in the smear. This article further discussed the significance of changes in LC morphology and density of the epidermis in the evaluation of cosmetic safety testing of weak allergens or weak primary irritants.