论文部分内容阅读
目的 研究肝纤维化大鼠模型 N-乙酰 -β-氨基葡萄糖苷酶 ( NAG)活性的变化及与羟脯氨酸 ( HYP)和透明质酸 ( HA)的相关性。方法 以四氯化碳建立大鼠肝纤维化模型 ,并用分光光度计比色法测定 NAG活性和HYP含量及放免法测定 HA含量。结果 大鼠肝纤维化造型过程中 ,肝组织 NAG含量明显升高 ( P <0 .0 5 ) ,尤以线粒体和溶酶体的 NAG活性升高更为显著 ( P <0 .0 1)。并发现 ,肝组织 NAG含量的升高与肝组织 HYP和 HA呈正相关 ( P <0 .0 1)。结论 NAG明显参与肝纤维化发病过程 ,其含量增加具有刺激和加速肝纤维化进程的作用
Objective To investigate the changes of N-acetyl-β-glucosaminidase (NAG) and the relationship with hydroxyproline (HYP) and hyaluronic acid (HA) in hepatic fibrosis rats. Methods Rat model of hepatic fibrosis was induced by carbon tetrachloride. NAG activity and HYP content were determined by spectrophotometer colorimetric assay and HA content was determined by radioimmunoassay. Results In the course of hepatic fibrosis, the content of NAG in liver tissues was significantly increased (P <0.05), especially in mitochondria and lysosomes (P <0.01). And found that liver tissue NAG content and liver tissue HYP and HA was positively correlated (P <0.01). Conclusion NAG is obviously involved in the pathogenesis of hepatic fibrosis, and the increased content of NAG has the effect of stimulating and accelerating the progression of hepatic fibrosis