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目的:探讨IL-4和TNF-α在肝纤维化中变化及意义。方法:二硝基硝胺(DMN)诱导大鼠肝纤维化模型,双抗夹心ELISA法测定血清白细胞介素4(IL-4),肿瘤坏死因子α(TNF-α),同时进行肝组织学检查和羟脯氨酸(Hyp)测定。结果:IL-4在肝纤维化中均逐步升高。TNF-α在肝纤维化中前期升高,后期降低。IL-4与肝纤维化组织Hyp的相关性较好,r分别为0.52。结论:IL-4、TNF-α参与肝纤维化形成的细胞因子网络的调节。
Objective: To investigate the changes and significance of IL-4 and TNF-α in liver fibrosis. Methods: The rat model of hepatic fibrosis induced by DMN was established. Serum interleukin 4 (IL-4) and tumor necrosis factor-α (TNF-α) were measured by double-antibody sandwich ELISA. Liver histology Examination and hydroxyproline (Hyp) assay. Results: IL-4 was gradually increased in liver fibrosis. TNF-α in pre-liver fibrosis increased later decreased. The correlation between IL-4 and Hyp in liver fibrosis was better, r = 0.52. Conclusion: IL-4 and TNF-α are involved in the regulation of cytokines in the formation of hepatic fibrosis.