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目的研究乙酰氧基胡椒酚乙酸酯(ACA)亚微乳和ACA原料药对HeLa细胞生长和凋亡的影响。方法用5、12.5、25、50、100、200μmol/L ACA亚微乳和ACA原料药处理HeLa细胞,MTT比色法检测ACA亚微乳和原料药作用24、48、72、96 h对HeLa细胞增殖的抑制作用;倒置显微镜观察HeLa细胞形态改变;流式细胞仪检测细胞凋亡和细胞周期。结果 ACA亚微乳和原料药均可明显抑制HeLa细胞增殖,其中ACA亚微乳的抑制效果较好;经ACA亚微乳和原料药处理的HeLa细胞显示典型的凋亡形态改变;HeLa细胞凋亡率与ACA处理时间、浓度呈正相关;ACA亚微乳和ACA原料药均使HeLa细胞阻滞于S期,且ACA亚微乳作用强于ACA原料药。结论与ACA原料药相比,ACA亚微乳抑制HeLa细胞增殖和诱导凋亡的作用更显著。
Objective To study the effects of acetoxy-pepperophenol acetate (ACA) submicroemulsion and ACA API on the growth and apoptosis of HeLa cells. Methods HeLa cells were treated with 5, 12.5, 25, 50, 100, 200μmol / L ACA submicroemulsion and ACA API. MTT colorimetric assay was used to detect the effect of ACA submicroemulsion and API on HeLa The cell proliferation was inhibited. The morphology of HeLa cells was observed by inverted microscope. The apoptosis and cell cycle were detected by flow cytometry. Results Both ACA submicroemulsion and drug substance could obviously inhibit the proliferation of HeLa cells, and the inhibitory effect of ACA submicroemulsion was better. The HeLa cells treated with ACA submicroemulsion and drug substance showed typical morphological changes of apoptosis. The apoptosis of HeLa cells ACA treatment time and concentration were positively correlated with mortality rate. Both ACA and ACA APIs blocked HeLa cells in S phase, and ACA submicroemulsion was stronger than ACA API. Conclusion Compared with ACA drug substance, ACA submicroemulsions can inhibit the proliferation and induce apoptosis of HeLa cells more significantly.