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目的探讨对可疑受精障碍的常规体外受精(IVF)周期在授精失败后补行卵胞浆内单精子注射(reacue ICSI)的时机及临床效果。方法对2006年1月至2007年6月在我中心接受常规IVF助孕治疗,可能有受精障碍的13例患者,取卵后6h授精,4h后观察,发现所有卵母细胞均未排出第二极体,立即行补救ICSI。结果13个周期共154个卵母细胞,其中MⅡ期142个;未行补救ICSI的46个均未受精;补救ICSI的96个,受精89个,受精率92.7%,移植13个周期29个胚胎,临床妊娠1例双胎和5例单胎,种植率24.1%。结论常规IVF受精后4h行补救ICSI可以获得较好的受精率和妊娠率。
Objective To investigate the timing and clinical effects of intracytoplasmic sperm injection (reacue ICSI) on routine in vitro fertilization (IVF) cycles of suspected infertility disorders after insemination. Methods From January 2006 to June 2007, routine IVF assisted pregnancy was performed in our center. Thirteen patients who may have impaired fertilization were fertilized at 6h after ovulation. After 4h, all the oocytes were not excreted Pole body, immediately relief ICSI. RESULTS: A total of 154 oocytes were found in 13 cycles, of which 142 were MⅡ, 46 of which were not fertilized, 46 of which were ICSI, 89 were fertilized, 92.7% of fertilization and 29 embryos of 13 cycles were transplanted , 1 case of twin pregnancy and 5 cases of singleton in clinical pregnancy, the planting rate was 24.1%. Conclusion ICSI can achieve better fertilization rate and pregnancy rate at 4h after IVF fertilization.