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以培养3周的新鲜人参愈伤组织为试材,分别进行5℃持续低温处理与25℃/5℃培养12h/12h间歇低温处理,取材时间为0、1、2、3、4d,分别设为CK、D1、D2、D3、D4处理,通过实时荧光定量PCR检测5种氧化鲨烯环化酶(OSC)基因在不同处理中的表达。结果表明:PgDAS的表达在持续式低温胁迫D4处理和间歇式低温胁迫D3处理中被显著诱导并达到峰值,分别为对照组的2.15、2.12倍;而Pgβ-AS1与Pgβ-AS2的表达仅在持续式低温胁迫D2处理中被诱导,分别为对照组的1.27、1.71倍;PgCAS与PgLOS的表达在2种低温胁迫中均未被诱导。表明PgDAS可能在响应不同低温胁迫时起到关键作用,而其它氧化鲨烯环化酶基因协同表达。
The fresh ginseng callus cultured for 3 weeks was used as the test material, and the cells were subjected to continuous low-temperature treatment at 5 ° C and intermittent low-temperature treatment at 25 ° C / 5 ° C for 12h / 12h, respectively. The extraction time was 0, 1, 2, The CK, D1, D2, D3 and D4 treatments were used to detect the expression of 5 oxidized squalene cyclase (OSC) genes in different treatments by real-time fluorescence quantitative PCR. The results showed that the expression of PgDAS was significantly induced and peaked in continuous cold stress D4 treatment and intermittent cold stress D3 treatment, which were 2.15 and 2.12 times as much as the control group, respectively. However, the expression of Pgβ-AS1 and Pgβ-AS2 The results showed that the expression of PgCAS and PgLOS was not induced in two kinds of low temperature stress in the treatment of continuous low temperature stress D2 treatment, which was 1.27 and 1.71 times of the control respectively. This suggests that PgDAS may play a key role in response to different cold stress while other squalene cyclase genes co-express.