【摘 要】
：
Coronavirus disease 2019 (COVID-19) is a new type of respiratory disease caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),which has caused severe social and economic costs and has become a great threat to public health because of the
【机 构】
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Beijing Institutes of Life Science,Chinese Academy of Sciences,Beijing 100101,China;Shaanxi Provinci
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Coronavirus disease 2019 (COVID-19) is a new type of respiratory disease caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),which has caused severe social and economic costs and has become a great threat to public health because of the high mortality rate.Recent studies indicated that respiratory pathogens co-infection and secondary infection were critical risk factors for the severity and mortality rates of COVID-19 (Zhou et al.,2020).Cur-rent laboratory-confirmed co-infections were those identified by pathogens culture,antigen detection,PCR detection and metatran-scriptomic sequencing.Among them,metatranscriptomic sequencing has a unique advantage due to its ability to detect all types of pathogens.This method typically starts with RNA fragmen-tation,followed by first-and second-strand cDNA synthesis,and then moves on to library preparation specified by high throughput sequencing technologies of choice (Chiara et al.,2021).However,most preparation protocols involve multiple costly and complicated steps,which inevitably affects the efficacy in the sequencing of time-sensitive SARS-CoV-2 samples and in the identification of other pathogens.For this reason,an easy,cost-effective,and relatively un-biased protocol for SARS-CoV-2 sample sequencing is needed to address this issue.
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