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研究了表面活性剂Tween80对有机氯农药林丹的增溶效果,对白腐真菌Phlebia brevispora及其胞内酶降解水溶液中林丹以及对菌株降解土壤中林丹的强化作用。结果表明,0.2~1.0 g/L的Tween80能大幅度增加林丹的溶解度,增溶倍数最高达到10.9。在0.4~1.0 g/L浓度范围内,Tween80能有效促进菌株对水相中林丹的降解,且降解率随着Tween80浓度的增加而增加。Tween80浓度为1.0 g/L时的林丹降解率达到最高的86.38%,比对照增加了18.88%。Tween80同样提高了菌株胞内酶对林丹的降解活性,在酶处理90 min后,添加0.4~1.0 g/L的Tween80处理时林丹的降解率显著高于对照处理(P<0.05)。添加了高浓度(0.6、0.8和1.0 g/L)Tween80的土壤体系中的林丹降解率则显著高于对照及低浓度处理(P<0.05)。尤其在添加1.0 g/L的Tween80的土壤中,处理30 d后菌株降解了77.69%的林丹,相比对照提高了14.27%。
The effect of surfactant Tween80 on the solubilization of organochlorine pesticide lindane was studied. The effect of lindane on degrading white soil fungus Phlebia brevispora and its intracellular enzyme-degrading solution and lindane on the degradation of soil were studied. The results showed that 0.2 ~ 1.0 g / L of Tween80 can significantly increase the solubility of lindane, solubilization fold up to 10.9. Under the concentration of 0.4-1.0 g / L, Tween80 could effectively promote the degradation of lindane in the aqueous phase, and the degradation rate increased with the increase of Tween80 concentration. When the concentration of Tween80 was 1.0 g / L, the degradation rate of lindane reached the highest 86.38%, an increase of 18.88% compared with the control. Tween80 also increased the degrading activity of lindane by intracellular enzymes. The degrading rate of lindane was significantly higher than that of the control (P <0.05) when treated with Tween80 at 0.4-1.0 g / L for 90 min. The degradation rates of lindane in soil systems with high concentration of Tween 80 (0.6, 0.8 and 1.0 g / L) were significantly higher than those of the control and low concentration treatments (P <0.05). Especially in the soil supplemented with 1.0 g / L of Tween80, the strain degraded 77.69% of lindane 30 d after treatment, which was 14.27% higher than that of the control.