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目的观察硫酸镁对小鼠肾缺血再灌注损伤的抗氧化保护作用。方法雄性小鼠随机分为假手术组、模型组和硫酸镁高、低剂量(120、60 mg/kg)组,无创动脉夹夹闭左肾蒂45 min和再灌注3 h制备急性肾缺血再灌注损伤模型,检测肾脏指数、血清尿素氮(BUN)和肌酐含量、肾组织丙二醛(MDA)含量以及超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活力,HE染色观察肾组织学变化。结果硫酸镁高、低剂量组小鼠肾指数分别为(0.72±0.05)和(0.74±0.07)、血清BUN含量为(12.36±2.24)和(15.58±1.92)mmol/L、血清肌酐水平为(98.23±4.37)和(114.63±6.24)μmol/L、肾组织MDA含量为(2.11±0.24)和(2.27±0.21)nmol/(mg.prot),肾组织SOD活力为(4.03±0.68)和(3.51±0.58)U/(mg.prot),硫酸镁高剂量组肾组织GSH-Px活力为(323.90±23.50)U/(mg.prot),与模型组比较,差异均有统计学意义(P<0.05),且肾组织病理变化较轻。结论硫酸镁对小鼠急性肾缺血再灌注损伤具有保护作用,其机制可能与抑制脂质过氧化反应有关。
Objective To observe the anti-oxidative effect of magnesium sulfate on renal ischemia-reperfusion injury in mice. Methods Male mice were randomly divided into sham operation group, model group and high and low doses of magnesium sulfate (60 and 60 mg / kg). Acute renal ischemia was established after 45 min reperfusion and 3 h reperfusion Reperfusion injury model, renal index, serum urea nitrogen (BUN) and creatinine, content of malondialdehyde (MDA) in renal tissue and the activity of superoxide dismutase (SOD) and glutathione peroxidase ) Vitality, HE staining observed renal histological changes. Results The renal index of high and low dose groups of magnesium sulfate were (0.72 ± 0.05) and (0.74 ± 0.07), the serum levels of BUN were (12.36 ± 2.24) and (15.58 ± 1.92) mmol / L, respectively The levels of MDA in renal tissue were (2.11 ± 0.24) and (2.27 ± 0.21) nmol / (mg.prot) respectively. The activities of SOD in renal tissue were (4.03 ± 0.68) and 3.51 ± 0.58) U / (mg.prot), the activity of GSH-Px in renal tissue of high-dose magnesium sulfate group was (323.90 ± 23.50) U / (mg.prot), and the difference was statistically significant <0.05), and pathological changes in renal tissue lighter. Conclusion Magnesium sulfate has a protective effect on acute renal ischemia-reperfusion injury in mice and its mechanism may be related to inhibition of lipid peroxidation.