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用组织化学方法结合体视学测量研究小鼠海马结构和大脑皮质胆碱能纤维的年龄变化以及长期适量运动（跑转笼）对胆碱能纤维的影响。以同年龄对照组心重／体重比率均值的２倍标准差作为运动有效标准。结果显示，老年鼠（２４月龄）海马ＣＡ１区、ＣＡ３区，齿状回分子层和运动、体感皮质第Ⅲ和Ⅴ层乙酰胆碱酯酶（ＡＣｈＥ）阳性纤维密度明显低于成年鼠（１３月龄）和青年鼠（５月龄）（Ｐ＜０．０１），后两者间除海马ＣＡ３区分子层纤维密度成年鼠稍低外Ｐ＜０．０５），其它各区无显著差异（Ｐ＞０．０５）；５月龄小鼠经过８和１９个月运动后，被检各区ＡＣｈＥ阳性纤维密度均显著高于同年龄对照组（Ｐ＜０．０１）。结果表明从青年开始的长期适量运动能够促进海马和皮质内胆碱能纤维的增生，延缓衰老时胆碱能纤维的丢失。
The changes of cholinergic fibers in hippocampal formation and cortex of mice were observed by histochemistry and stereology. The standard deviation of 2 times the mean of body weight / body weight ratio of the same age control group was taken as the effective standard of exercise. The results showed that the acetylcholinesterase (AChE) positive fibers in hippocampus CA1, CA3, dentate gyrus and motor and somatosensory cortex in aged mice (24 months old) were significantly lower than those in adult mice (13 months old ) And young mice (5 months old) (P <0.01), while there was no significant difference between the other two groups except for the lower fiber density in the hippocampus CA3 subfields (P> 0.05) After 5 and 5 months of age, the AChE - positive fibers in all the tested areas were significantly higher than those of the same age group (P <0.01) after 8 and 19 months of exercise. The results showed that long-term moderate exercise started from the youth can promote the proliferation of alkaline fiber in the hippocampus and cortex, and delay the loss of cholinergic fibers during aging.