论文部分内容阅读
青霉素类药物的薄层层析的检出试剂,具有特异性差,或虽有特异性但不够灵敏,且操作不简便的缺点。本文报导了应用氯铂酸与青霉素中的硫醚基反应,可作为一灵敏而有特异性的试剂,快速简便地检出薄层层析中各种青霉素及与青霉素有关的物质。测定时样品液需新鲜配备,将样品溶解于水、缓冲液或有机溶媒,配制成10或100微克毫升的溶液,准确点样1～10微升于硅胶G或纤维素薄板上,按下述溶剂系统进行单向薄层层析:(1)正丁醇-乙醇-水(12∶8∶2);(2)正丁醇-乙醇-乙酸-水(50∶15∶15∶20);(3)正丁醇-乙酸-水(60∶15∶25),展开10厘米距离,待层析板完全干燥后,以氯铂酸试剂喷雾检出斑点。
Penicillins drugs TLC detection reagent, with poor specificity, or although not specific enough but not sensitive, and the shortcomings of the operation is not easy. This paper reports the use of chloroplatinic acid and penicillin thioether reaction, can be used as a sensitive and specific reagents, rapid and easy detection of penicillin in thin layer chromatography and penicillin-related substances. The sample solution should be freshly prepared. When the sample is dissolved in water, buffer or organic solvent, prepare a solution of 10 or 100 micrograms milliliter, accurately spot 1-10 microliters on silica gel G or cellulose sheet, The solvent system was subjected to unidirectional thin layer chromatography: (1) n-butanol-ethanol-water (12:8: 2); (3) n-butanol - acetic acid - water (60:15:25), expand the distance of 10 cm, until the chromatography plate is completely dry, chloroplatinic acid reagent spray spot detection.