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利用 4个分属宗亲群 4,7,1 7和 44的菌株连续接种在C0 39的单基因近等基因系和基因累加系上 ,以获得突变抗病基因新菌株。经 1 3轮接种循环 ,共分离到 9个新菌株。其中 8个针对C1 0 1LAC(Pi- 1 )突变 ,另 1个针对C1 0 1PKT (Pi- 4a)和F1 2 8(Pi-ta2 )的双位点突变。 4个原始菌株 ,P0 6 - 6 ,92 48- 6 ,92 39- 4和 1 0 1 - 7-2的突变频率分别为 :0 ,2 82× 1 0 -5,2 0 5× 1 0 -5和 9 0 9× 1 0 -5。菌株针对不同抗病基因的突变频率不同 ,由Av- 1变到Av - 1 + 和由Avir - 4a(t)突变到Av - 4a+ 的频率分别为 7 5 1× 1 0 -5和 6 1 4× 1 0 -5。没有针对Pi- 2突变体出现。
Four strains belonging to clans 4, 7, 17 and 44 belonging to clan 4 were inoculated continuously on the single-gene near-isogenic lines and gene accumulation lines of C0 39 to obtain new strains of the mutant disease-resistance genes. After 13 rounds of inoculation, a total of 9 new strains were isolated. Eight of them were for the C1 0 1 LAC (P-1) mutation and the other one for the two-site mutation of C1 0 1 PKT (Pi- 4a) and F1 2 8 (Pi-ta2). The mutation frequency of four original strains, P0 6 - 6, 92 48 - 6, 92 39 - 4 and 1 0 1 - 7-2 were 0,282 × 10 -5, 2 0 5 × 10 4 - 5 and 9 0 9 × 10 -5. The frequency of mutation of Av - 1 to Av - 1 + and the mutation of Avir - 4a (t) to Av - 4a + was 751 × 10 -5 and 6 1 4, respectively. × 1 0 -5. No P-2 mutant appeared.