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以通辽市科左后旗查金台牧场野生大豆和栽培品种大白眉种间杂交后代为材料,通过SRAP分子标记鉴定其真实性并构建杂交后代指纹图谱,首先从234对引物中筛选出15对扩增条带较多、信号强、背景清晰的引物组合,再利用2个亲本对引物进行筛选,筛选出10对能扩增出父本特异性条带的引物。对50个杂交后代进行真实性鉴定,鉴定结果为50个杂交后代中有2个后代未扩增出父本特征带,被鉴定为假杂种。用筛选出的15对引物组合对大豆各株系进行PCR扩增,共扩增出347个位点,其中有265个为多态性位点,多态性位点的百分率为76.37%。结果表明,SRAP分子标记可以用于鉴定大豆杂交后代的真实性,所筛选出的15对引物组合可以有效地应用于杂交大豆种质资源的SRAP分析。
Using SRAP molecular markers to identify their authenticity and the fingerprints of hybrid progenies, the cross between the wild and cultivated white-browed wild soybean cultivars in Chaqintai pasture in Kezuohou Banner, Tongliao City, Tongliao City, was screened out from 234 pairs of primers The primers with more amplification bands, stronger signal and clear background were screened by using two parents, and 10 pairs of primers that could amplify the male-specific bands were screened out. The authenticity of 50 hybrid progenies was identified. The results showed that two out of 50 hybrid progenies did not amplify the male-derived bands and were identified as fake hybrids. A total of 347 loci were amplified by PCR with 15 selected primer combinations. Among them, 265 were polymorphic and the percentage of polymorphic loci was 76.37%. The results showed that the SRAP marker could be used to identify the authenticity of the progeny of soybean cross. The 15 primer combinations screened by SRAP could be effectively applied to SRAP analysis of hybrid soybean germplasm resources.