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本实验研究TY-51469对疲劳运动引起小鼠巨噬细胞格局变化的调节作用。选用健康雄性(6~8)周龄C57BL/6小鼠,分为安静对照组、运动组、运动+TY组。运动组及运动+TY组进行2周平板跑步运动,前3d为适应性运动,以后每日逐渐增加运动量,运动至小鼠普遍出现疲劳征象。第5d起运动+TY组每日运动后按15mg/kg腹腔注射TY-51469,安静对照组及运动组第5d起每天腹腔注射等体积PBS,一共注射8d。运动结束后取脾脏制作单个核细胞悬液标记巨噬细胞后进行流式细胞检测,并提取巨噬细胞基因测定IRF5和IRF4mRNA表达量,观察并分析疲劳运动对脾脏M1、M2型巨噬细胞数量及比例的影响,以及TY-51469对疲劳运动引起巨噬细胞格局变化的调节作用。实验结果显示,疲劳运动可导致小鼠脾脏巨噬细胞M1/M2比值上升,TY-51469通过恢复M1/M2比例起到对巨噬细胞不同亚群的调节作用,使巨噬细胞格局恢复正常。
In this study, TY-51469 on fatigue caused by exercise-induced macrophage pattern of regulatory changes. Healthy male (6-8) weeks old C57BL / 6 mice were divided into quiet control group, exercise group and exercise + TY group. Exercise group and exercise + TY group for 2 weeks running treadmill exercise, the first three days for the adaptive exercise, gradually increased daily exercise, exercise to mice generally appear fatigue signs. The rats in exercise + TY group were injected intraperitoneally with TY-51469 at 15mg / kg after exercise for 5 days, and the rats in control group and exercise group were injected intraperitoneally with equal volume of PBS for 5 days. After the exercise, the spleen was taken out to make mononuclear cell suspension labeled macrophages and flow cytometry was performed. The expression of IRF5 and IRF4 mRNA in macrophages was extracted and the numbers of M1 and M2 macrophages in spleen were observed and analyzed. And the impact of TY-51469 on the regulatory effect of fatigue-induced changes in macrophages. Experimental results show that fatigue exercise can lead to an increase in the M1 / M2 ratio of mouse splenic macrophages. TY-51469 regulates macrophages by restoring M1 / M2 ratio and normalizes the pattern of macrophages.