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目的:探讨印楝油复合物对小鼠的抗生育作用。方法:昆明种雌性小鼠分别每日灌胃含1ml/kg、1.5ml/kg和2ml/kg的印楝油复合物的1%羧甲基纤维素(CMC),灌胃1周后,与正常性成熟雄鼠合笼。雌鼠继续受试至19d,剖检受试雌鼠,统计受孕率,并采集卵巢、子宫和肝脏、脾脏、心脏、肺脏、肾脏组织固定,进行病理学研究,计算小鼠脾脏脏器指数和肉芽肿。同时进行抗着床、抗早孕、抗中期和抗晚期试验。所有试验均设对照值,相应地给予等量的1%CMC灌胃。结果:印楝油复合物具有显著的抗着床和抗早孕作用,较好的抗中期和晚期妊娠作用。对卵巢和子宫有一定程度的损伤,卵泡细胞发生变性、坏死、炎性细胞浸润,子宫炎性水肿,大量白细胞渗出。心、肺和肾无明显病变,肝和脾组织中大量巨噬细胞浸润。对脾脏脏器指数影响显著,具有一定的抗炎作用。结论:印楝油复合物具有明显的抗生育作用,其作用机制一方面可能是通过影响卵泡细胞的代谢过程,导致代谢障碍而致使卵泡细胞发生变性、坏死;另一方面,可能与局部细胞免疫介导有关。
Objective: To investigate the anti-fertility effects of neem oil complex on mice. METHODS: Kunming female mice were intragastrically administered with 1% carboxymethyl cellulose (CMC) containing 1 ml/kg, 1.5 ml/kg, and 2 ml/kg of neem oil complexes once daily for 1 week. Normally mature male mice were caged. The female rats continued to be tested until 19 days. The test females were examined by necropsy to count the conception rate. Ovary, uterus and liver, spleen, heart, lung and kidney tissues were collected for pathological study. The mouse spleen organ index was calculated and calculated. Granuloma. At the same time, anti-implantation, anti-pregnancy, mid-resistance and anti-advanced tests were performed. All experiments were set to control values and correspondingly given equal amounts of 1% CMC gavage. Results: Neem oil complex has significant anti-implantation and anti-pregnancy effects, and better anti-middle and late pregnancy effects. There is a certain degree of damage to the ovaries and the uterus. The follicular cells undergo degeneration, necrosis, inflammatory cell infiltration, inflammatory edema of the uterus, and exudation of large numbers of leukocytes. There were no obvious lesions in the heart, lungs and kidneys, and a large number of macrophages infiltrated in the liver and spleen. It has a significant effect on spleen visceral index and has a certain anti-inflammatory effect. Conclusion: The neem oil complex has obvious anti-fertility effects. Its mechanism may be that it may affect the metabolic process of follicular cells and lead to metabolic disorders, resulting in degeneration and necrosis of follicular cells; on the other hand, it may be related to local cellular immunity. Mediation related.