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目的研究雄芍汤抗大鼠免疫性肝纤维化的作用。方法 84只SD大鼠随机分为对照组、模型组、扶正化瘀胶囊阳性对照组、雄芍汤预防组和雄芍汤高、低剂量(生药15.8、7.9 g/kg)组(造模后给药)。采用ip猪血清法制备大鼠肝纤维化模型,观察雄芍汤对肝纤维化大鼠血清肝功能指标丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、白蛋白(ALB)、白蛋白与球蛋白比值(A/G)和肝纤维化血清标志物透明质酸(HA)、层粘连蛋白(LN)、III型前胶原(PCIII)、IV型胶原(CIV)水平,以及肝组织病理学的影响。结果与模型组比较,雄芍汤各给药组血清ALT、AST、HA、LN、PCIII水平明显降低(P<0.05、0.01),血清ALB水平和A/G值明显升高(P<0.01);雄芍汤预防组血清CIV水平明显降低(P<0.05);所有给药组肝细胞变性和坏死程度轻微,肝组织纤维增生较少,雄芍汤高剂量组和预防组的作用尤为明显。结论雄芍汤能减轻免疫性肝纤维化大鼠的肝细胞损伤,改善肝功能,调节细胞外基质的代谢,阻止或逆转肝纤维化。
Objective To study the effect of XiongShao decoction on immune hepatic fibrosis in rats. Methods Eighty-four SD rats were randomly divided into control group, model group, positive control group of Fuzhenghuayu capsule, XiongShao-Tang preventive group and XiongShao-Tang high-dose and low-dose group (crude drug 15.8 and 7.9 g / kg) medicine). The rat model of hepatic fibrosis was established by ip pig serum method. The effect of XiongShao-tao on serum hepatic function indicators such as ALT, ALB, Albumin to globulin ratio (A / G), and liver fibrosis serum markers hyaluronic acid (HA), laminin (LN), type III procollagen (PCIII), type IV collagen (CIV) Histopathological effects. Results Compared with the model group, serum ALT, AST, HA, LN and PCIII levels in all the three groups were significantly decreased (P <0.05, 0.01), serum ALB levels and A / G values were significantly increased (P <0.05). The serum levels of CIV in the Xiong Shao Tang prevention group were significantly lower than those in the control group (P <0.05). The degree of degeneration and necrosis of hepatocytes in all administration groups was slight, and the fibrosis of liver tissue was less. Conclusion Xiong Shao-Tang can relieve hepatic injury, improve liver function, regulate extracellular matrix metabolism and prevent or reverse liver fibrosis in immune-induced hepatic fibrosis rats.