Inhibiting severe acute respiratory syndrome-associated coronavirus by small interfering RNA

• 作 者: 张仁礼 郭中敏 陆家海 孟锦绣 周灿权 詹希美 黄冰 余新炳 黄民 潘兴华 凌文华 陈系古 万卓越 郑焕英 鄢心革 王一飞 冉延超 刘新健 马俊鑫 王承宇 张必良
• 机 构: Sun Yat-Sen University,Sun Yat-Sen University,Sun Yat-Sen University,Sun Yat-Sen University,Sun Yat-
• 出 处: Chinese Medical Journal
• 发表日期: 2003年08期
• 关键词: small interfering RNA severe acute respiratory syndrome coronavirus cytopathic e

Objective To evaluate the effectiveness of small interfering RNA (siRNA) on inhibiting severe acute respiratory syndrome (SARS)-associated coronavirus replication,and to lay bases for the future clinical application of siRNA for the treatment of viral infectious diseases.Methods Vero-E6 cells was transfected with siRNA before SARS virus infection,and the effectiveness of siRNA interference was evaluated by observing the cytopathic effect (CPE) on Vero-E6 cells.Results Five pairs of siRNA showed ability to reduce CPE dose dependently,and two of them had the best effect. Conclusion siRNA may be effective in inhibiting SARS-associated coronavirus replication.

Objective To evaluate the effectiveness of small interfering RNA (siRNA) on inhibiting severe acute respiratory syndrome (SARS) -associated coronavirus replication, and to lay bases for the future clinical application of siRNA for the treatment of viral infectious diseases. Methods Vero-E6 cells was transfected with siRNA before SARS virus infection, and the effectiveness of siRNA interference was evaluated by observing the cytopathic effect (CPE) on Vero-E6 cells. Results Five pairs of siRNA showed ability to reduce CPE dose dependently, and two of them had the best effect. Conclusion siRNA may be effective in inhibiting SARS-associated coronavirus replication.