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目的:研究新城疫病毒(NDV)对肿瘤细胞的作用。方法:采用空斑试验观察NDV对鸡胚成纤维细胞(CEF)的作用,通过细胞抑制试验、琼脂糖凝胶电泳、TUNEL染色、细胞骨架染色及细胞唾液酸含量测定等,观察NDV对肿瘤细胞的影响。结果:NDV对CEF、HeLa及Hep-2等肿瘤细胞可产生明显的病变,而对人的羊膜细胞(Wish细胞)无明显影响;对Hela细 胞和Hep-2细胞能产生明显的生长抑制作用,但无量效依赖关系。NDV还可导致以凋亡为主的细胞死亡,引起Hela细胞和Hep-2细胞的细胞骨架发生改变,以及去除肿瘤细胞表面唾液酸的作用。结论:NDV能选择性地作用于人肿瘤细胞,抑制肿瘤细胞的生长,导致肿瘤细胞发生凋亡,有望成为一种有效的抗肿瘤生物制剂。
Objective: To study the effect of Newcastle disease virus (NDV) on tumor cells. Methods: The effect of NDV on chicken embryo fibroblasts (CEF) was observed by plaque test. The effect of NDV on tumor cells was observed by cytostatic assay, agarose gel electrophoresis, TUNEL staining, cytoskeleton staining and cell sialic acid assay. Impact. Results: NDV had obvious pathological changes on CEF, HeLa and Hep-2 tumor cells, but had no obvious effect on human amniotic cells (Wish cells), and had obvious growth inhibitory effects on Hela cells and Hep-2 cells. But the ineffective dependency. NDV can also lead to the death of apoptotic cells, resulting in changes in the cytoskeleton of Hela cells and Hep-2 cells, as well as the removal of sialic acid on the surface of tumor cells. Conclusion: NDV can selectively act on human tumor cells, inhibit the growth of tumor cells and lead to the apoptosis of tumor cells, which is expected to become an effective anti-tumor biological agent.