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目的:分析独活的挥发油成分。方法:利用水蒸气蒸馏法提取独活挥发油,用气质联用法检测,对重叠色谱峰采用子窗口因子分析法进行分辨,从而获得每一组分的纯色谱和质谱,依靠每一组分纯质谱在NIST质谱库进行相似性检索而定性分析,用总体积积分法进行定量分析。结果:从独活挥发油样品中共分离出67个色谱峰,鉴定出其中53个组分,占挥发油总含量的85.86%,主要组分为4-甲氧基-6丙烯基-1,3苯并二噁茂(16.96%)、α-红没药醇(9.3%)、(+)-环异酒剔烯(6.83%)、1-[3-3-甲氧基-5-羟苯基]-1,2,3,4-四甲基异喹啉(6.73%)、棕榈酸(4.36%)、β-倍半水芹烯(3.92%)、茄酮(3.74%)和4-羟基-3-甲基苯乙酮(2.78%)。结论:该方法不仅可使鉴定的化合物数目增加,而且提高了定性准确度,可用于中药独活质量控制,能为进一步研究和开发独活提供有利的科学依据。
Objective: To analyze the composition of live-volatile oil. Methods: The essential oil was extracted by steam distillation and detected by gas chromatography-mass spectrometry (GC-MS). The overlapping chromatographic peaks were resolved by the sub-window factor analysis to obtain the pure chromatograms and mass spectra of each component. NIST mass spectral library for qualitative search and qualitative analysis, the total volume integral method for quantitative analysis. Results: Sixty-seven chromatographic peaks were isolated from the volatile oil samples. Fifty-three components were identified, accounting for 85.86% of the total volatile oil. The main components were 4-methoxy-6-propenyl-1,3benzodi (16.96%), α-bisabolol (9.3%), (+) - cyclohexylidecinolide (6.83%), 1- [3-3-methoxy-5-hydroxyphenyl] - 1,2,3,4-tetramethylisoquinoline (6.73%), palmitic acid (4.36%), β-secresol (3.92%), solanone (3.74%) and 4-hydroxy-3 -methylacetophenone (2.78%). Conclusion: This method not only can increase the number of identified compounds, but also improve the qualitative accuracy. It can be used for the quality control of independent Chinese medicines and can provide favorable scientific basis for further research and development of independent living.