反向毛细管电泳法测定不同产地远志中细叶远志皂苷的含量(英文)

• 作 者: 郝增燕 王洁 张一鸣 陈安家
• 机 构: 山西医科大学药学院,
• 出 处: Journal of Chinese Pharmaceutical Sciences
• 发表日期: 2011年04期
• 关键词: 远志皂苷 细叶 反向毛细管电泳 methanol 毛细管电泳分离 细叶远志皂苷 Radix 分离条件 毛细管电泳法 硼砂缓冲液

首次建立了简单、经济、有效的反向毛细管电泳分离测定远志药材中细叶远志皂苷含量的方法。通过对分离条件的选择优化,采用含有35%甲醇(v/v)的50mmol/L的硼砂缓冲液使待测成分得到完全分离;细叶远志皂苷在0.0563-0.9000mg/mL浓度范围内呈良好线性关系,r=0.9988,加样回收率在99.50%-104.7%之间。结果表明该方法简便、快速、准确可靠,可作为远志的质量控制方法。

For the first time established a simple, economical and effective reverse capillary electrophoresis separation Polygalaceae Polygonatum saponin content method. By optimizing the separation conditions, the analytes were completely separated by using 50 mmol / L borax buffer containing 35% methanol (v / v). The concentration of Saponin in the concentration range of 0.0563-0.9000 mg / mL Linear relationship, r = 0.9988, sample recovery rate between 99.50% -104.7%. The results show that the method is simple, rapid, accurate and reliable, and can be used as the quality control method of Polygala.