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目的通过对动脉粥样硬化(AS)兔血清和粥样硬化斑块中纤溶酶原激活物抑制物-1(PAI-1)表达的研究,探讨PAI-1在AS中的作用。方法16只雄性大耳白兔随机分为正常饮食组和高脂饮食组,每组8只,饲养16周。两组白兔均于0周、16周取耳缘静脉血,检测血清中PAI-1的水平;16周后处死,应用免疫组化方法检测PAI-1在主动脉粥样硬化斑块中的表达。结果正常饮食组和高脂饮食组0周血清中PAI-1水平差异无显著性(P>0.05);高脂饮食组16周后血清PAI-1水平较0周显著增加(P<0.01);免疫组化结果显示高脂饮食组主动脉壁PAI-1的表达明显高于对照组(P<0.01)。结论动脉粥样硬化的发生伴有血清和粥样斑块中PAI-1表达增加,PAI-1可能参与了AS的形成。
Objective To investigate the effect of PAI-1 on the expression of plasminogen activator inhibitor-1 (PAI-1) in atherosclerotic (AS) rabbit serum and atherosclerotic plaque. Methods Sixteen male large white rabbits were randomly divided into normal diet group and high fat diet group, with 8 rabbits in each group and fed for 16 weeks. The rabbits in both groups were taken at the 0th week and the 16th week to collect the level of PAI-1 in the serum. After 16 weeks, the rabbits were killed and the expression of PAI-1 in the aortic atherosclerotic plaque was detected by immunohistochemistry expression. Results There was no significant difference in serum PAI-1 level between the normal diet group and the high fat diet group at 0 week (P> 0.05). After 16 weeks of high fat diet group, serum PAI-1 level increased significantly compared with 0 week group (P <0.01). The results of immunohistochemistry showed that the expression of PAI-1 in the aortic wall of the high-fat diet group was significantly higher than that of the control group (P <0.01). Conclusions Atherosclerosis is associated with an increase in PAI-1 expression in serum and atherosclerotic plaques, and PAI-1 may be involved in the formation of AS.