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急性淋巴细胞白血病(ALL)初诊和复发时有抗原受体基因结构的改变,许多报道表明ALL复发时有IgH重链基因重排的缺失或新的重排出现。作者进一步研究了ALL初诊和复发时T细胞受体γ(TCRG)基因的结为,分析了ALL初诊和复发时白血病克隆的改变。 在24例ALL病人中,13例为cALL,1例为前B ALL,2例力null-ALL,8例为T-ALL。所用方法为:多聚酶链反应(PCR)扩增重排的TCRG基因的V-J片段,将PCR产物及酶切产物进行琼脂糖电泳分析,并将PCR产物克隆,测序,同时用Southern
Acute lymphoblastic leukemia (ALL) at the time of initial diagnosis and recurrence of antigen receptor gene structure changes, many reports indicate ALL recurrence of IgH heavy chain gene rearrangement deletion or new rearrangement. The authors further studied the T cell receptor gamma (TCRG) gene junction in newly diagnosed and relapsed ALL patients and analyzed the changes in leukemia clones at the initial and relapse of ALL. Among the 24 ALL patients, 13 were cALL, 1 was pre-B ALL, 2 were null-ALL, and 8 were T-ALL. The method used was: polymerase chain reaction (PCR) amplification of rearranged TCRG gene V-J fragment, the PCR products and digested products were subjected to agarose electrophoresis analysis, and PCR products were cloned, sequenced, while using Southern