论文部分内容阅读
In order to know the specific immume responses after immunization of HBV DNA vaccines and what kind differences of immune responses following the HBV pan-S gene and HBsAg gene vaccination,the DNA vaccines,both pcDNA3-PS and pcDNA3-HBsAg,in which HBV pan-S gene and HBsAg gene sequences were inserted into downstream of CMV promoter in pcDNA3,respectively,were injected intramuscularly into BALB/C mice.Specific humoral and cellular immune responses were detected after Vaccination.The serum anti-HBs were detected in all of the pcDNA3-PS and the pcDNA3-HBsAg immunized mice,but none in control mice.However,there were no significantly differences of serums anti-HBs level between the pcDNA3-PS and pcDNA3-HBsAg immunized mice.Serum levels of interleukine 2 and interferon gamma,were also significantly higher in pcDNA3-PS and pcDNA3-HBsAg immunized mice than that in controls.It was indicated that the good responses of cellular and humoral immunity can be induced by injection of pbDNA3-PS and pcDNA3-HBsAg in BALB/C mice.No any evidence was showed that HBV pan-S gene vaccine could enhance the immunity of HBsAg gene vaccine.
In order to know the specific immume responses after immunization of HBV DNA vaccines and what kind of differences of immune response following the HBV pan-S gene and HBsAg gene vaccination, the DNA vaccines, both pcDNA3-PS and pcDNA3-HBsAg, in which HBV pan -S gene and HBsAg gene sequences were inserted into downstream of CMV promoter in pcDNA3, respectively, were injected intramuscularly into BALB / C mice. Specific humoral and cellular immune responses were detected after Vaccination. The serum anti-HBs were detected in all of the pcDNA3-PS and the pcDNA3-HBsAg immunized mice, but none in control mice. Having, no significant differences of serums anti-HBs level between the pcDNA3-PS and pcDNA3-HBsAg immunized mice. Levels of interleukine 2 and interferon gamma , was also significantly higher in pcDNA3-PS and pcDNA3-HBsAg immunized mice than that in controls. It was indicated that the good responses of cellular and humoral immunity can be induced by injection of pbDNA3-PS and pcDNA 3-HBsAg in BALB / C mice. No any evidence was showed that HBV pan-S gene vaccine could enhance the immunity of HBsAg gene vaccine.