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目的 :建立人侵蚀性葡萄胎裸鼠皮下移植瘤模型 ,探讨其生物学特性。方法 :将人侵蚀性葡萄胎的新鲜组织块种植于裸鼠背部皮下 ,在无特殊病原菌 (SPF)条件下饲养裸鼠 ,原代生长后鼠间传代 ,观察移植瘤的生长特点 ,观察其组织形态学和超微结构 ,检测血中人绒毛膜促性腺激素 ( β -hCG)水平 ,利用流式细胞仪 (FCM)测定DNA含量 ,分析染色体核型。结果 :经 19个月建成人侵蚀性葡萄胎裸鼠皮下移植模型HIM - 980 1,瘤株生长稳定 ,已传代 54代。自第 2代起 ,移植成瘤率均为 10 0 % ,移植瘤潜伏期及传代间期短 ,生长速率快 ,向周围组织浸润 ,光镜和电镜下的形态学特征与患者肿瘤组织一致 ,DNA检测和染色体核型分析为非整倍体。结论 :HIM -980 1是首次建立成功的人侵蚀性葡萄胎裸鼠皮下移植瘤模型 ,为深入研究此病提供了理想的材料和动物体内瘤株。
OBJECTIVE: To establish a human subcutaneous xenograft tumor model of human invasive hydatidiform mole and investigate its biological characteristics. Methods: Fresh tissue explants of human invasive hydatidiform mole were implanted subcutaneously in the back of nude mice. Nude mice were housed in the absence of special pathogens (SPF), and passaged after primary growth. The growth characteristics of the transplanted tumor were observed. Morphology and ultrastructure were observed. The level of human chorionic gonadotropin (β -hCG) in blood was detected. The DNA content was determined by flow cytometry (FCM) and the karyotype was analyzed. Results: HIM - 980 1, a subcutaneous transplant model of human invasive hydatidiform mole, was established in 19 months. The tumor grew steadily and had been passaged for 54 passages. Since the second generation, the rate of tumorigenesis was 100%. The engraftment period and passage time of transplanted tumor were short, the growth rate was fast and infiltrated to the surrounding tissues. The morphological features under light and electron microscopes were consistent with the tumor tissues of the patients. DNA Detection and chromosome karyotype analysis for aneuploidy. Conclusion: HIM-9801 is the first successful model of human subcutaneous xenografts in nude mice with invasive hydatidiform mole. It provides an ideal material and animal tumor in-depth study of this disease.