论文部分内容阅读
应用色谱分离理论，综合考虑了分子扩散、进样量、能量耗散等因素对高效毛细管电泳分离的影响，建立了高效毛细管电泳分离模型。研究了电压、溶液浓度、进样量、毛细管管径等因素对理论极高度的影响，并与模型计算结果进行了比较。结果表明，所提出的模型能够较好地描绘高效毛细管电泳的分离机理，该模型可作为优化操作条件和进行放大设计的基础。在此基础上，应用高效毛细管电泳技术对生物产品进行了分离，以探讨该技术在生物领域的应用。
Based on the theory of chromatographic separation, the effects of molecular diffusion, injection volume, energy dissipation and other factors on high performance capillary electrophoresis separation were comprehensively considered, and a high performance capillary electrophoresis separation model was established. The effects of voltage, solution concentration, injection volume, capillary diameter and other factors on the theoretical maximum height were studied and compared with the results of model calculation. The results show that the proposed model can better depict the separation mechanism of high performance capillary electrophoresis. The model can be used as the basis for optimizing the operating conditions and for the magnified design. On this basis, the application of high performance capillary electrophoresis separation of biological products to explore the technology in the field of biological applications.