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目的研究断乳后不同饲料构成对大鼠成年后摄入高脂膳食时体脂含量及脂肪组织中激素敏感性脂肪酶基因表达的影响。方法新生Wistar雄性大鼠24天断乳,按体重随机分为A、B、C、D4组,分别给予高碳水化合物构成的基础饲料、高蛋白质构成的饲料、高不饱和脂肪和高饱和脂肪构成的饲料喂养3周。然后均转为基础饲料喂养2周后,按体重将A组随机分为A1、A2两组。A1组继续喂饲基础饲料,A2、B、C、D4组则给予高脂饲料喂养6周,结束实验。动态观察大鼠的体重、体脂含量和血糖变化,并应用RT-PCR法动态检测白色脂肪组织中HSLmRNA水平。结果实验末期,断乳后喂饲高不饱和脂肪构成饲料的C组,体重、体脂含量和血糖均显著低于高脂对照组A2(P<0.05);断乳后喂饲高蛋白质的B组体重和体脂含量明显低于A2组(P<0.05),但血糖水平高于A2(P>0.05);断乳后摄取高饱和脂肪饲料的D组,尽管体重明显低于A2(P<0.05),但血糖和体脂含量与A2组无差异(P>0.05)。动态观察发现,断乳后喂饲高不饱和脂肪的C组大鼠,白色脂肪组织HSL基因表达水平持续升高。结论断乳后喂饲高不饱和脂肪饲料不仅可以降低高脂膳食大鼠的体重、体脂含量,还可明显改善葡萄糖代谢异常,是较为合理的断乳后饮食;对基因表达的调控可能参与早期饮食对成年肥胖的程序性影响。
Objective To study the effects of different diet compositions after weaning on body fat content and hormone-sensitive lipase gene expression in adipose tissue of adult rats fed with high fat diet. Methods The newborn Wistar male rats were weaned on 24th day and were randomly divided into groups A, B, C and D4 according to body weight. The rats were given basic diet composed of high carbohydrate, diet composed of high protein, high unsaturated fat and high saturated fat Feed for 3 weeks. Then were converted to basal diet for 2 weeks, according to the weight of A group were randomly divided into A1, A2 two groups. Group A1 continued to feed basal diet, and groups A2, B, C and D4 were given high-fat diet for 6 weeks to finish the experiment. Dynamic changes of body weight, body fat content and blood glucose were observed. HSLmRNA levels in white adipose tissue were detected by RT-PCR. Results At the end of the experiment, the body weight, body fat content and blood glucose in group C fed diets containing high unsaturated fat were significantly lower than those in high-fat control group A2 (P <0.05). After weaning, high-protein B Body weight and body fat content were significantly lower in group A2 than those in group A2 (P <0.05), but their blood glucose levels were higher than those in group A2 (P> 0.05). Group D received high saturated fat diet after weaning, although the body weight was significantly lower than A2 (P < 0.05), but the blood sugar and body fat content had no difference with A2 group (P> 0.05). Dynamic observation found that HSA gene expression in white adipose tissue increased continuously in group C fed with high level of unsaturated fat after weaning. Conclusion The feeding of high unsaturated fat diet after weaning can not only reduce the body weight and body fat content of high-fat diet rats, but also can significantly improve the abnormal glucose metabolism, which is a reasonable post-weaning diet. The regulation of gene expression may be involved The Programmatic Effects of Early Diet on Adult Obesity.