Effect of antisense transfecting of monocarboxylate transporter gene on biological characteristics o

• 作 者: ZHANG Gui-zhi HUANG Gui-jun GUO Xian-jian QIAN Gui-sheng
• 机 构: Institute of Respiratory Diseases, Xinqiao Hospital, Third Military Medical University, Chongqing 40
• 出 处: 中国人民解放军军医大学学报
• 发表日期: 2002年2期
• 关键词: monocarboxylate transporter gene gene transfecting pHi lactate lung neoplasm

Objective: To study the influence of transfecting antisense expression vector of the first subtype of the monocarboxylate transporter (MCT1) gene into lung cancer cells on pHi regulation, lactate transportation and cell growth, Methods: MCT1 antisense gene recombinant vector was introduced into human lung cancer cell line A549 by electroporation. The transfected A549 cells resistant to G418 were selected. Positive clones were examined by using PCR. The changes of intracellular pH and lactate were examined with spectrophotometric method. Cell growth was studied with cell growth curve. Results: Intracellular pH and lactate were remarkably decreased in the cells transfected pLXSN-MCT1 in comparison with A549 cells without transfection (P＜0. 001). The growth of A549 cells transfected pLXSN-MCT1 was also inhibited remarkably. Conclusion: MCT1 gene may play an important role in pHi regulation, lactate transportation and cell growth in tumor cells.