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近年来,提出了关于二硫化碳(CS_2)毒性作用机制的新假设。作者曾指出,在慢性中毒过程中,CS_2毒性作用的主要因素之一是V_(B6)的缺乏。本研究的目的在于寻求接触CS_2对组织中V_(B6)的含量和依赖V_(B6)代谢反应的影响程度。对CS_2影响的估价是根据组织中磷酸吡哆醛含量的测定;依赖于V_(B6)的酶活性的测定,和用生化试验证明吡哆醇的缺乏(色氰酸负荷试验和尿中4-吡哆酸的测定)。受试大鼠连续接触CS_21～2.5天(急性接触),或者慢性地接触数月(缓慢间歇性接触)。这些动物用不同含量的V_(B6)的食物饲养。
In recent years, a new hypothesis on the mechanism of toxicity of carbon disulfide (CS 2) has been proposed. The authors have pointed out that in the process of chronic poisoning, CS2 toxicity is one of the main factors V_ (B6) lack. The purpose of this study was to investigate the effect of exposure to CS_2 on the V_ (B_6) content and the V_ (B_6) metabolic response in tissues. The assessment of the effect on CS_2 is based on the determination of the pyridoxal phosphate content in the tissue; the enzyme activity dependent on the V_ (B6) assay and the biochemical test to demonstrate the absence of pyridoxine (Cyanocysteine loading test and urinary 4- Determination of pyridoxine). Subjects were consecutively exposed to CS 21 to 2.5 days (acute exposure) or to chronic exposure to chronic (slow intermittent exposure). These animals were fed with different levels of V_ (B6).