半薄切片的一种多色染色法

• 作 者: 史颖辉 刘健
• 机 构: 第四军医大学电镜室,第四军医大学电镜室
• 出 处: 第四军医大学学报
• 发表日期: 1992年03期
• 关键词: 半薄切片 光学显微镜检查 碱性复红法 多色染色 超薄切片 苏木素 天青Ⅱ 美蓝 硼酸钠 加拿大树胶

作超薄切片前,一般要先切成0.5～1.0μm厚的切片称半薄切片,染色后用光学显微镜观察定位。半薄切片的染色法目前已有多种,如美蓝-天青Ⅱ法、碱性复红法、苏木素-伊红法等。这些方法或者染出的颜色单一,或者程序较多、所需时间长。Chien法虽无上述缺点,但染片容易出现过染,常呈现一片红色,分色也不易控制。我们对上述方法做了改进,染色效果明显改善。

For the ultra-thin section, the general first cut into 0.5 ~ 1.0μm thick slices called semi-thin sections, stained with a light microscope to observe the location. Semi-thin sections of the staining method currently has a variety of, such as the United Blue - Azure II method, alkaline complex red method, hematoxylin - Eosin law. These methods either dyed a single color, or the program more long time required. Chien method although without the above shortcomings, but the dye is prone to over-dyeing, often showing a red, color separation is not easy to control. We have made improvements to the above method, dyeing results improved significantly.