论文部分内容阅读
【目的】明确辽宁省辣椒疫病菌多态性及致病力分化与区域性关系。【方法】利用SRAP技术对辽宁省25个辣椒疫病菌菌株进行了PCR扩增及NTSYS-PC聚类分析,用灌根法进行致病力分化试验并对试验结果进行SPSS 11.5分层聚类分析。【结果】利用筛选出的27组引物对25个菌株进行扩增,得到578条条带,每对引物多态性比率在84%?100%之间,多态性丰富;供试菌株间遗传相似性较高,相似系数0.56?0.91,以相似系数0.68为阈值划分,25个菌株可聚为4组。试验菌株80%为中等致病力,聚类结果较为分散。【结论】供试菌株没有表现出明显的区域性特征,菌株致病力强弱分化区域特征性规律不明显。
【Objective】 The objective of this study was to determine the polymorphism and virulence of pepper phytophthora blight in Liaoning Province. 【Method】 PCR-amplified PCR and NTSYS-PC cluster analysis of 25 capsici in Liaoning Province were carried out by using SRAP technique. The pathogenic differentiation test was carried out by the method of root-filling, and the results were analyzed by SPSS 11.5 stratified cluster analysis . 【Result】 A total of 578 bands were amplified using the 27 primers selected. The polymorphic ratio of each primer pair ranged from 84% to 100% with rich polymorphism. The similarity coefficient was 0.56? 0.91, with the similarity coefficient of 0.68 as the threshold value, and 25 strains could be grouped into 4 groups. 80% of the test strains were moderately pathogenic and the clustering results were more dispersed. 【Conclusion】 The tested strains did not show obvious regional characteristics, and the pathogenicity of the virulent strains was not obvious.