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探讨4种常见纳米材料(单壁碳纳米管,多壁碳纳米管,纳米银,量子点)对小鼠骨代谢的影响.选取8周龄健康昆明小鼠给予不同剂量的上述4种纳米材料,各纳米材料分别设高、中、低剂量组,溶剂对照、离子对照组,以及阳性对照组,每周3次进行腹腔注射.用药6周后检测骨破坏情况、骨生物力学改变以及血清中钙的含量.结果显示,4种纳米材料与其相应溶剂对照组相比,多壁碳纳米管溶骨发生率显著增高,高、中剂量组骨灰干重比及3个剂量组生物力学指标有显著性差异,高剂量组血清钙显著升高;单壁碳纳米管溶骨发生率上升,高、中剂量组骨灰干重比及生物力学指标降低,各组血清钙水平无显著性差异;量子点各剂量组溶骨发生率无差异,高、中剂量组骨灰干重比、最大载荷、弹性载荷降低,血清钙水平无显著性差异;纳米银各剂量组溶骨发生率未增加,灰干重比、生物力学各指标及血清钙水平无统计学差异.多壁碳纳米管长期暴露可引起小鼠溶骨性破坏、骨量下降、骨强度减低;单壁碳纳米管与量子点对骨代谢的影响不显著,纳米银不会引起骨代谢改变.
The effects of four kinds of common nanomaterials (single-walled carbon nanotubes, multi-walled carbon nanotubes, nanosilver, quantum dots) on bone metabolism in mice were studied.An eight-week-old Kunming mice were given different doses of the above four nanomaterials , Each nanomaterial was divided into high, medium and low dose groups, solvent control, ion control group, and positive control group, intraperitoneal injection of three times a week.After 6 weeks of treatment, bone destruction, bone biomechanical changes and serum Calcium content.The results showed that compared with the corresponding solvent control group, the incidence of osteolytic osteoclasts in multi-walled carbon nanotubes was significantly increased in the four nanomaterials, and the dry matter weight-to-weight ratio in the high and middle dose groups and the biomechanical indexes in the three dose groups There was no significant difference in the level of serum calcium between the high dose group and the high dose group. The incidence of osteolytic bone of single-walled carbon nanotubes increased, while the dry weight ratio and biomechanical index of high and medium dose groups decreased. There was no difference in the rate of osteolytic bone in each dose group. The dry weight ratio, the maximum load, the elastic load and the serum calcium level of high and medium dose groups had no significant difference. The incidence of osteolytic bone in each dose group did not increase, Biomechanics There was no significant difference between each index and serum calcium level. Long-term exposure to multi-walled carbon nanotubes caused osteolytic destruction, decreased bone mass and decreased bone strength in mice. Single-walled carbon nanotubes and quantum dots had no significant effect on bone metabolism , Nano-silver does not cause changes in bone metabolism.