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目的 观察绿色荧光灯持续照射后大鼠视网膜损伤的病理形态变化特征。方法 采用 (190 0± 10 6 .9)lux绿色荧光灯对SD大鼠进行持续 2 4h照射。分别于光照前 ,光照后 6h、6d及 14d进行视网膜光镜和电镜形态学观察、外核层厚度检测。结果 光照前视网膜形态正常 ,结构层次清楚 ,内外节排列整齐规则。光照后 6h视网膜外核层变薄 ,其厚度减少 2 3 .91% ,内外节排列紊乱 ;光感受器细胞核肿胀 ,内节线粒体肿胀 ,外节水肿 ,RPE顶端微绒毛消失 ,溶酶体增多。光照后 6d外核层更薄 ,其厚度减少 46 .6 % ,损伤加重。 14d外核层较 6d增厚 ,其厚度减少 42 .4%。光感受器细胞及内节已基本正常 ,外节再生但盘膜排列稀疏 ,RPE顶端出现微绒毛。结论 本实验条件下 ,大鼠视网膜光化学损伤的病理特征是退行性变性过程
Objective To observe the pathological changes of retinal damage in rats after continuous green fluorescent lamp irradiation. Methods SD rats were irradiated with (190 ± 10 6. 9) lux green fluorescent light for 24 hours. Retinal light microscopy and electron microscopy morphological observation, outer nuclear layer thickness were performed before light, 6h, 6d and 14d after light. The results of the normal retinal light before the light, clear structure, internal and external arrangement of neat rules. The outer retinal layer became thinner after 6 hours and its thickness decreased by 23.91%. The inner and outer segments were disordered. The nucleus of photoreceptors swollen, the inner mitochondria swelled, the outer edema disappeared, and the top of the RPE disappeared and lysosomes increased. The outer nuclear layer was thinner 6 days after light exposure, the thickness of which was reduced by 46.6% and the damage was aggravated. 14d outer nuclear layer thicker than 6d, the thickness decreased by 42.4%. Photoreceptor cells and internal segments have been normal, external regeneration, but the disc is arranged sparsely, RPE top microvilli. Conclusion Under the experimental conditions, the pathological features of rat retinal photochemical damage are degenerative processes