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信号识别颗粒受体在分泌性蛋白合成和分泌过程中起重要作用。微管蛋白对细胞内各种生命活动都是必需的。将数据库中花生抗黄曲霉和敏感品种的两类α-微管蛋白α和α7和SR蛋白进行序列比较分析发现,在种子发育早期5,α-微管蛋白在敏感品种各有三条EST,抗性品种各有一条EST,晚期7只有抗性品种各有一条EST。SR只在抗性品种的6、7时期各有一条EST,敏感品种中没有。用荧光定量PCR方法对抗性品种KB153与敏感品种JH1012发育中不同时期的果实和部位进行差异表达分析,结果表明在果实发育早期的小果期,SR、α-微管蛋白α和α7在抗性品种中都显著上调表达,说明SR介导的内质网蛋白质运输途径与黄曲霉抗性有关。SR基因在抗性品种的子叶中的表达也上调,这与抗性品种中一些贮藏蛋白含量尤其是蛋白酶抑制剂高于敏感品种的现象一致。
Signal recognition Particle receptors play an important role in the synthesis and secretion of secreted proteins. Tubulin is essential for a variety of life activities in the cell. Comparison of the two α-tubulin α and α7 and SR proteins in the peanut anti-aflatoxin and susceptible cultivars showed that in the early seed development stage, α-tubulin had three ESTs in sensitive varieties There is one EST for each of the sex breeds, and one for each of the seven late resistant varieties. SR only had one EST in 6 and 7 resistant varieties, but none in susceptible varieties. The differential expression analysis of the resistant and susceptible varieties KB153 and the susceptible cultivar JH1012 in different developmental stages by fluorescence quantitative PCR showed that the SR, α-tubulin α and α7 were resistant in the early fruit development stage Significantly up-regulated in all the cultivars showed that SR-mediated endoplasmic reticulum protein transport was associated with aflatoxin resistance. The SR gene was also up-regulated in the cotyledons of resistant cultivars, consistent with some of the storage protein content of the resistant cultivars, especially the higher protease inhibitors than the susceptible cultivars.