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目的:观察小鼠视网膜片层化过程中胶质细胞增殖及与双极细胞分化的关系。方法:各个年龄小鼠共计123只。应用免疫荧光和HE染色法对各个年龄的小鼠视网膜形态结构及胶质细胞的增殖、分化进行观察。结果:星形胶质细胞按照离心型的模式发育,P0时仅在视乳头和邻近周围区域出现幼稚的星形胶质细胞,尔后细胞突起相互缠绕形成的网状联系中出现中空“管样”结构,细胞形态呈现典型的星状外形,类似血管网的脉络。P6时小鼠视网膜Müller细胞零星的表达GS,双极细胞也开始表达少量的PKC-α,尔后Müller细胞GS阳性表达逐渐增加,而PKC-α阳性细胞伸向节细胞层的轴突更加密集,树突和胞体的数量也增多,P14时双极细胞的发育过程基本完成。同时,小鼠视网膜GS和PKC-α双标记,发现Müller细胞内、外突上发出许多细小的侧突与双极细胞的胞体相接触,提示Müller细胞在双极细胞的分化、迁移过程中伴着很重要的角色。结论:在小鼠视网膜片层化过程中,胶质细胞起着关键性作用,Müller细胞在双极细胞的分化、迁移过程中起重要作用。
OBJECTIVE: To observe the relationship between glial cell proliferation and bipolar cell differentiation in retinal laminarization in mice. Methods: A total of 123 mice of all ages. Immunofluorescence and HE staining were used to observe the morphological changes of retina and the proliferation and differentiation of glial cells in all age groups. Results: The astrocytes developed in a centrifugal mode. At P0, naive astrocytes appeared only in the optic disc and the surrounding area. After that, the astrocytes "Structure, cell morphology presents a typical star-shaped appearance, similar to the context of vascular network. P6 mouse retinal Müller cells sporadic expression of GS, bipolar cells also began to express a small amount of PKC-α, then Müller cells GS positive expression increased gradually, and PKC-α positive cells extending toward the ganglion cell layer more dense axons, The number of dendrites and cell bodies also increased. The development of bipolar cells at P14 was basically completed. At the same time, double labeling of mouse retina GS and PKC-α found that many small lateral processes of Müller cells were in contact with the cell bodies of bipolar cells, suggesting that Müller cells are involved in the process of bipolar cell differentiation and migration A very important role. Conclusion: Glial cells play a key role in retinal laminarization in mice. Müller cells play an important role in the differentiation and migration of bipolar cells.