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为探究大豆木质素含量及合成关键基因在与大豆疫霉菌互作过程中的变化趋势及相关性,利用大豆疫霉菌株P6497接种大豆品种Williams,分别于6,8,10和15 d分析了根部组织木质素含量及合成相关基因的表达。结果表明:根组织中木质素含量在疫霉菌侵染过程中积累速度显著增加;对木质素合成过程中9个合成关键基因表达模式分析表明,PAL、C4H和F5H表达水平在所选4个时间点均显著高于未接种对照;CCo AOMT在接种后第8,10和15天3个时间点的表达水平显著高于对照;4CL的表达在接种后第10和15天时比对照上调超过100倍;CAD和CCR表达水平分别在接种后8和10 d与对照存在显著差异;C3H和COMT的表达量在处理组和对照组中差异不显著。在接种样品中,C4H、4CL和CCR基因表达与木质素含量变化模式呈显著正相关。以上结果表明:大豆根部木质素积累受疫霉侵染诱导,暗示木质素参与大豆与疫霉互作过程,同时根据试验结果推测C4H、4CL和CCR是诱导木质素含量提高的关键基因。
In order to explore the change trend and correlation between the content of lignin and key synthetic genes of soybean and Phytophthora sojae, soybean was inoculated with Phytophthora sojae P6497 to analyze the root at 6, 8, 10 and 15 days Tissue lignin content and expression of genes involved in the synthesis. The results showed that the accumulation rate of lignin in root tissues increased significantly during the infection of Phytophthora infestans. The analysis of the expression patterns of nine key synthetic genes during the synthesis of lignin showed that the expression levels of PAL, C4H and F5H were significantly increased at four selected times CCo AOMT was significantly higher than the control at 3 time points on the 8th, 10th and 15th day after inoculation. The expression of 4CL was up-regulated more than 100-fold on the 10th and 15th day after inoculation compared with the control The expression levels of CAD and CCR were significantly different from the control at 8 and 10 days after inoculation. The expression levels of C3H and COMT were not significantly different between the treated group and the control group. In inoculation samples, C4H, 4CL and CCR gene expression and lignin content was significantly positively correlated with the pattern. The above results showed that the accumulation of lignin in soybean roots was induced by Phytophthora infestans, suggesting that lignin was involved in the interaction between soybean and Phytophthora capsici. At the same time, C4H, 4CL and CCR were the key genes that induced the increase of lignin content.