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以霜霉病抗性葡萄品种左优红和感病品种霞多丽为材料,研究硫化氢(H2S)在葡萄应答霜霉病过程中的作用及其与过氧化氢(H2O2)的相互关系。结果表明,接种霜霉病菌后,抗性品种左优红和感病品种霞多丽叶片均出现H2S含量的猝发、H2S合成酶基因表达量增加、抗病相关蛋白多酚氧化酶(PPO)和β-1,3葡聚糖酶(Glu)活性显著升高现象;且H2S清除剂次牛磺酸(HT)可以显著抑制霜霉病菌诱导的PPO和Glu活性升高,并导致葡萄的感病率和病情指数显著升高;同时外源H2S可降低两个葡萄品种的感病率和病情指数。说明,H2S是参与葡萄抗霜霉病过程的新的信号物质。受霜霉病菌侵染后,葡萄叶片H2O2含量猝发早于H2S,并且H2O2清除剂抗坏血酸(AsA)可显著抑制霜霉病菌所诱导的H2S含量的上升,而H2S清除剂HT对霜霉病菌诱导的H2O2含量变化影响不显著,推测,H2S作用于H2O2的下游参与葡萄抵御霜霉病过程。
The effects of hydrogen sulfide (H2S) on grape downy mildew and its relationship with hydrogen peroxide (H2O2) were studied using the Zygoderm-resistant grape variety Zou You Hong and the susceptible cultivar Chardonnay. The results showed that after inoculation with downy mildew, the resistant varieties Zuyouhong and susceptible Chardonnay leaves showed a burst of H2S content, H2S synthase gene expression increased, the disease-associated protein polyphenol oxidase (PPO) and beta-1, 3-glucanase (Glu) activity was significantly increased. H 2 S scavenger hypotaurine (HT) significantly inhibited the activity of PPO and Glu induced by downy mildew and induced the susceptibility of grape Rate and disease index increased significantly; at the same time exogenous H2S can reduce the susceptibility and disease index of two grape varieties. H2S is a new signal substance involved in the process of grape downy mildew resistance. After inoculation by downy mildew, H2O2 content in grape leaves was earlier than that of H2S, H2S scavenger ascorbic acid (AsA) significantly inhibited the increase of H2S level induced by downy mildew, and H2S scavenger HT induced by downy mildew H 2 O 2 content had no significant effect. It was speculated that H2S played a role in downstream resistance of H2O2 to resist downy mildew.