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以普通小球藻(Chlorella vulgaris)为受试生物,采用批量培养法考察了不同质量浓度的硼酸和四硼酸钠对藻细胞生物量、ρ(Chla)、ρ(蛋白质)以及MDA(丙二醛)含量的96 h急性毒性效应,初步探讨了硼化合物对普通小球藻的毒性作用.结果表明:在2种硼化合物胁迫下藻细胞的生长受到抑制,尤其是当ρ(硼)≥40 mg/L时抑制作用极其显著(P<0.01);ρ(硼)为0~80 mg/L时,硼酸和四硼酸钠对藻细胞生长的最大抑制率分别为89.42%和86.72%,硼的抑制作用呈明显的剂量-效应关系,并且随暴露时间延长而减弱,96 h时藻细胞恢复生长.硼酸和四硼酸钠对藻细胞的96 h-EC_(50)(半数有效浓度)分别为82.03和71.19 mg/L(以硼计);与对照组相比,二者对普通小球藻的毒性效应表现为,随着暴露时间的延长,ρ(Chla)降低、ρ(蛋白质)先减后增、MDA含量升高.研究显示,硼化合物对普通小球藻的毒性作用,可能是由于短期内引发藻细胞产生活性氧自由基并造成膜脂质和其他生物大分子的氧化损伤所致.
Using Chlorella vulgaris as tested organism, the effects of different concentrations of boric acid and sodium tetraborate on the biomass, ρ (Chla), ρ (protein) and MDA (malondialdehyde ) 96h poisoning effect, the toxic effects of boron compounds on Chlorella vulgaris were preliminarily studied.The results showed that the growth of algal cells was inhibited under the stress of two boron compounds, especially when ρ (boron) ≥40 mg (P <0.01). The maximum inhibitory rates of boric acid and sodium tetraborate on algae cell growth were 89.42% and 86.72%, respectively, at ρ (0 ~ 80 mg / L) The dose-effect relationship was obvious, and with the prolongation of exposure time, the algae cells regained their growth after 96 hours.The rates of 96 h-EC 50 (half effective concentration) of boric acid and sodium tetraborate to algal cells were 82.03 and 71.19 mg / L (calculated as boron); compared with the control group, the toxic effects of the two on Chlorella vulgaris were: ρ (Chla) decreased and ρ (protein) , MDA content increased.Studies show that the toxicity of boron compounds on Chlorella vulgaris may be due to the short-term initiation Cells produce reactive oxygen species and oxidative damage caused by membrane lipids, and other biological macromolecules caused.