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目的:观察中药复方861对二乙基亚硝胺(diethylnitrosamine,DEN)诱癌过程的影响。方法:250只雄性SD大鼠随机分成模型组、干预组、正常对照组。模型组和干预组以1%DEN灌胃,干预组同时以中药复方861灌胃。实验开始后分别于第2、3、5、8、10、12、14、18周分批处死大鼠,观察肝脏外观,肝组织进行HE、Masson三色染色,并对炎症坏死、肝纤维化程度和卵圆细胞增生程度进行评分,应用免疫组化及Western-blot方法检测肝组织中胎盘型谷胱甘肽硫转移酶(GST-P)蛋白的表达,计算成癌率。结果:干预组比模型组肝脏炎症坏死程度(P<0.01)、纤维化程度(P<0.01)和卵圆细胞增生程度(P<0.05)轻;GST-P蛋白表达量少。结论:中药复方861可减轻DEN所致肝损伤。
Objective: To observe the effect of Chinese herbal compound 861 on carcinogenesis of diethylnitrosamine (DEN). Methods: 250 male SD rats were randomly divided into model group, intervention group and normal control group. The model group and the intervention group were intragastrically administrated with 1% DEN, while the intervention group was given intragastric administration of Chinese herbal compound 861 at the same time. The rats were sacrificed in batches on the 2nd, 3rd, 5th, 8th, 10th, 12th, 14th and 18th week respectively after the start of the experiment. The appearance of the liver was observed. The liver tissues were subjected to HE and Masson trichrome staining. The necrosis and hepatic fibrosis The degree and the degree of oval cell proliferation were scored. The expression of placental glutathione S-transferase (GST-P) in liver tissue was detected by immunohistochemistry and Western-blot, and the rate of carcinogenesis was calculated. Results: Compared with model group, the degree of hepatic inflammation and necrosis (P <0.01), the degree of fibrosis (P <0.01) and the degree of oval cell proliferation (P <0.05) were lighter in the intervention group than in the model group. The expression of GST-P protein was less. Conclusion: Chinese herbal compound 861 can reduce the liver damage caused by DEN.