Down-regulation of Coxsakie and Adenovirus Receptor during Embryo Implantation

• 作 者: Yufeng LI Huan ZHAO Beibei WANG Dandan CUI Suzhen YUAN Xiao HE Na GUO Ningning CHEN
• 机 构: Reproductive Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Scienc
• 出 处: 华中科技大学学报（医学）（英德文版）
• 发表日期: 2012年1期
• 关键词: coxsackie and adenovirus receptor embryo implantation endometrium

In this study,real-time PCR and immunohistochemistry were used to detect coxsakie and adenovirus receptor (CAR) expression.Both localization and quantity were evaluated in the uteri obtained at days post coitus (dpc) 2.5,4.5,6.5,8.5.Outcome of PCR was assessed by 2-△△Ct method.Image Pro-Plus 6.0 software was used for quantifying mean density of CAR expression in immunohistochemical sections.We found relatively weak CAR expression in the mouse uteri during implantation window.PCR and immunohistochemistry revealed highest CAR expression was detected on dpc 2.5 followed by down-regulation of CAR at dpc 4.5 and 6.5 (with significant difference).At dpc 8.5,CAR expression was increased slightly again.It is concluded that during implantation,the expression of CAR mRNA and protein is declined,resulting in the impairment of tight junction between cavity epithelium cells.After implantation window closure,CAR appears again to maintain epithelium stability.CAR might play an important role during embryo implantation procedure.