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目的通过荧光定量PCR法定位检测性病后慢性前列腺炎的解脲脲原体,为其诊治提供参考依据。方法共收集性病后慢性前列腺炎43例,按照“四杯法”原理收集尿液和前列腺液及前列腺按摩前的尿道试子标本,分别做前列腺液常规、尿道试子及前列腺液的解脲脲原体荧光定量PCR检测,对比前列腺按摩前、后标本中解脲脲原体拷贝数来进行病原体的定位。结果 43例患者中有2例解脲脲原体阳性,检出率为4.65%。结论性病后慢性前列腺炎中解脲脲原体的检出率较低,但其仍可能参与发病。
Objective To detect Ureaplasma urealyticum (Ureaplasma urealyticum) of chronic prostatitis by fluorescence quantitative PCR method and provide a reference for its diagnosis and treatment. Methods A total of 43 cases of chronic prostatitis were collected after sexually transmitted diseases. Urethra and prostatic fluid and prostatic urethra specimens were collected according to the “Four-cup method” principle. Prostate fluid routine, urethral prostatic fluid and prostatic fluid solution Ureaplasma urealyticum quantitative real-time PCR detection, before and after prostate massage in specimens of Ureaplasma urealyticum copy number for pathogen localization. Results 2 of 43 patients were positive for Ureaplasma urealyticum, the detection rate was 4.65%. Conclusion The detection rate of Ureaplasma urealyticum in chronic prostatitis is low, but it may still be involved in the pathogenesis.