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近年来许多学者认为厚涂片查痰能提高抗酸杆菌的检出率。我们用100例住院肺结核病人的痰液比较了厚涂片,直接涂片(薄片)和漂浮集菌涂片的检出阳性率。材料与方法一、标本制作:取结核病人晨痰3～5毫升,挑取标本中灰白色细丝及干酪样颗粒物,于洁净载物片上,涂布20×25mm~2的范围,按许敏媛的方法涂两张厚片,24小时痰液高压蒸汽灭菌的漂浮集菌涂片和直接涂片各一张。二、染色方法:皆以萋纳氏法染色。直
In recent years, many scholars believe that thick smear sputum can improve the detection rate of acid-fast bacilli. We compared sputum from 100 sputum samples of hospitalized tuberculosis patients with thick smear, direct smear (flake) and floating bacterial smear detection positive rate. Materials and methods First, the specimen production: Take sputum sputum 3 to 5 ml of tuberculosis patients, pick the specimens in the gray filament and cheese-like particles on the clean carrier tablets, coating 20 × 25mm ~ 2 range, according to Xu Minyuan method Coated with two thick films, 24 hours of sputum autoclaving floating bacterial smear and direct smear each one. Second, the staining method: all by 萋 Na’s method of staining. straight