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目的 观察套细胞淋巴瘤的临床病理学特征及细胞周期蛋白D1染色在诊断中的意义。方法 对 8例淋巴结套细胞淋巴瘤作临床病理观察及随访 ,LSAB法做免疫表型分析 (CD4 5RO、CD5、CD2 0、细胞周期蛋白D1、Ki 6 7、bcl 2 )。结果 患者年龄 4 3～ 78岁 (平均年龄 5 7岁 ) ,男女 3∶1。组织学特点为 :(1)淋巴结结构破坏并被单一的淋巴样细胞所取代 ,淋巴细胞以套区增生性、结节性、弥漫性三种模式增生。 (2 )淋巴样细胞胞核有一定的不规则性 ,染色质中等致密 ,核分裂象少见 ,类似中心细胞。其中有 3例转变为高度侵袭性的母细胞样变型。所有的病例都呈cyclinD1与bcl 2阳性、CD2 0阳性、CD4 5RO阴性、CD5阳性。结论 套细胞淋巴瘤有其特征性的形态改变及免疫表型。根据组织病理学特征及cyclinD1阳性 ,可与其他类型的小B细胞淋巴瘤相鉴别。套细胞淋巴瘤的母细胞样变型也应当与其他变型区别。
Objective To investigate the clinicopathological features and the significance of cyclin D1 staining in mantle cell lymphoma. Methods Eight cases of lymph node mantle cell lymphoma were observed clinically and pathologically. LSAB method was used to analyze the immunophenotype (CD4 5RO, CD5, CD20, cyclin D1, Ki67, bcl2). Results Patients aged 4 to 78 years (mean age 5 7 years), men and women 3: 1. Histological features: (1) destruction of lymph node structure and was replaced by a single lymphoid cells, lymphocytes to nest area proliferative, nodular, diffuse three models of hyperplasia. (2) Lymphoid cells have some irregular nuclei, chromatin medium density, rare mitotic figures, similar to the central cells. Three of these were converted to highly aggressive somatic variants. All cases were cyclinD1 and bcl2 positive, CD20 positive, CD4 5RO negative, CD5 positive. Conclusion Mantle cell lymphoma has its characteristic morphological changes and immunophenotypes. According to histopathological features and cyclinD1 positive, with other types of small B cell lymphoma phase identification. The soma-like variant of mantle cell lymphoma should also be distinguished from other variants.