哺乳动物心脏中发现内源性类洋地黄活性因子:Cardiodigin

• 作 者: 赵凯存
• 出 处: 国外医学.药学分册
• 发表日期: 1983年06期
• 关键词: 活性因子 Cardiodigin 心肌细胞 内源 治疗作用 强心甙 大鼠 狄戈辛 提取物 放射免疫分析

1953年Georgyi曾提出在正常生物体内可能存在一种内源性因子,心力衰竭患者可能由于缺乏这种因子而致病,由此提示,强心甙的治疗作用是由于能模拟这种因子的作用。最近,在豚鼠、牛和大鼠的心脏中发现存在内源性类洋地黄活性因子,并命名为Cardiodigin。用0.32 M蔗糖溶液将动物心脏磨成匀浆,在低渗条件下使心肌细胞破裂,离心,活性因子存在于去细胞

1953 Georgyi has proposed that there may be an endogenous factor in normal organisms, heart failure patients may be due to the lack of such factors and pathogenic, suggesting that the therapeutic effect of cardiac glycosides is due to be able to mimic the role of this factor . Recently, the presence of endogenous digitalis-like activity factor was found in the hearts of guinea pigs, cattle and rats and was named Cardiodigin. The heart of the animal was homogenized with 0.32 M sucrose solution and the cardiomyocytes were dissected under hypotonic conditions and centrifuged. The active factor was present in acellular