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[目的]研究UHRF1在膀胱癌中的表达及临床意义。[方法]采用实时荧光定量逆转录聚合酶链反应(RT-PCR)和免疫组化法分别检测58例膀胱癌组织和20例相应正常组织中UHRF1的表达,并分析表达的差异与临床病理特征的关系。[结果]UHRF1 mRNA在膀胱癌中的表达量(107.0±24.3)高于正常组织的表达量(1.9±0.4),差异有显著统计学意义(P<0.001);免疫组化显示UHRF1蛋白在膀胱癌组织中的阳性表达率为53.4%,正常组织中未见表达,两种组织的表达有明显差异(P<0.001)。UHRF1蛋白的表达与肿瘤分级(P=0.026)和分期(P=0.017)有关,与性别、年龄、肿瘤大小、数目无明显相关性(P>0.05)。[结论]UHRF1的表达在肿瘤组织中明显高于正常组织,且与病理分级、临床分期有关,UHRF1的过表达可能促进膀胱癌的进展。
[Objective] To investigate the expression and clinical significance of UHRF1 in bladder cancer. [Method] The expression of UHRF1 in 58 cases of bladder cancer and 20 cases of corresponding normal tissues were detected by real-time fluorescence quantitative reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry. The difference of expression and clinicopathological features Relationship. [Results] The expression of UHRF1 mRNA in bladder cancer (107.0 ± 24.3) was significantly higher than that in normal tissues (1.9 ± 0.4) (P <0.001). Immunohistochemistry showed that the expression of UHRF1 protein in bladder The positive expression rate in cancer tissues was 53.4%, which was not found in normal tissues. There was significant difference between the two tissues (P <0.001). The expression of UHRF1 protein was correlated with tumor grade (P = 0.026) and stage (P = 0.017), but not with gender, age, tumor size and number (P> 0.05). [Conclusion] The expression of UHRF1 in tumor tissue was significantly higher than that in normal tissue, and was related to the pathological grade and clinical stage. The overexpression of UHRF1 may promote the progression of bladder cancer.