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目的:初步探讨Bmi-1基因对胶质瘤细胞凋亡状况的影响。方法:采用siRNA技术干扰沉默U251胶质瘤细胞中Bmi-1基因,RT-PCR法检测干扰效果,流式细胞仪检测U251细胞的凋亡状况,one way ANOVA和t检验进行统计学分析。结果:RT-PCR显示U251细胞RNA干扰组Bmi-1 mRNA凝胶电泳条带呈弱阳性,阴性对照组和未处理组Bmi-1 mRNA条带呈强阳性,灰度比统计分析,差异具有统计学意义,siRNA可以明显降低U251细胞中Bmi-1基因的表达;流式细胞仪显示干扰组U251细胞的凋亡率为(32.53±6.33)%,高于阴性对照组的凋亡率(21.91±5.63)%,统计分析其差异具有统计学意义。结论:Bmi-1基因可以抑制胶质瘤细胞的凋亡,有可能促进了胶质细胞瘤的发生、发展。
Objective: To investigate the effect of Bmi-1 gene on the apoptosis of glioma cells. Methods: siRNA was used to interfere with the silencing of Bmi-1 gene in U251 glioma cells. RT-PCR was used to detect the interference effect. Flow cytometry was used to detect the apoptosis of U251 cells. One way ANOVA and t test were used for statistical analysis. Results: RT-PCR showed that Bmi-1 mRNA bands in U251 cells were weakly positive, while Bmi-1 mRNA bands in negative control group and untreated group were strongly positive. The difference was statistically significant In the sense of significance, siRNA could significantly decrease the expression of Bmi-1 gene in U251 cells. Flow cytometry showed that the apoptosis rate of U251 cells was (32.53 ± 6.33)%, higher than that of the negative control group (21.91 ± 5.63)%, statistical analysis of the difference was statistically significant. Conclusion: Bmi-1 gene can inhibit the apoptosis of glioma cells, which may promote the occurrence and development of glioma.