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本试验选用了1480条RAPD引物,以26份国内外亚麻种质资源或品种为材料,进行了引物筛选。筛选出了32条多态性比较好的引物,32个RAPD标记共产生79个多态性条带,多态性比率为44.4%。并利用这32条引物对26份材料进行了遗传多样性分析,利用UPGMA法做聚类图,计算其遗传相似系数。将26份材料分为3个类群。在筛选出的32个RAPD引物中选出多态性好,特异性好的10个核心引物,编号分别为:S1047、S1230、S1238、S1270、S1314、S1353、S2105、SC07、SH04、ST09。利用这10个核心引物构建了26份亚麻材料的DNA指纹图谱,并将条带的有或无转化成1或0,每份材料构成了一组71位数二进制数据,将每份材料的二进制数据转换为22位的十进制数据,构成了每份材料的分子身份证,初步建立亚麻RAPD标记分子身份证体系。
In this experiment, 1480 RAPD primers were used in this study, and 26 domestic and foreign flax germplasm resources or varieties were selected as the materials for primer selection. Thirty-two polymorphic bands were screened out with 32 RAPD markers, of which 32 were polymorphic. The polymorphism rate was 44.4%. The genetic diversity of 26 cultivars was analyzed using these 32 primers. The UPGMA method was used to do cluster analysis to calculate the genetic similarity coefficient. The 26 materials were divided into three groups. Among the 32 RAPD primers screened, 10 core primers with good polymorphism and good specificity were selected and identified as S1047, S1230, S1238, S1270, S1314, S1353, S2105, SC07, SH04 and ST09. Using these 10 core primers, DNA fingerprinting of 26 flaxseeds was constructed and the presence or absence of bands was converted to 1 or 0, each consisting of a set of 71-digit binary data. The binary The data is converted into 22-bit decimal data, which constitutes the molecular ID for each material, with the initial establishment of a flax RAPD tagged molecular ID card system.